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40 times refers to the number of duplication cycles during the Reaction. You first have a sample, wash it, purify it and add chemicals to multiply the DNA strands you are looking for. Since DNA is of very low concentration in the sample, you gotta make more of it to have detectable amounts in your end product. Each of the 40 cycles effectively doubles the amount of DNA, because during one cycle one strand of DNA gets split and the halves (mono-strands) are each completed using free bases which you added during preparation.
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[–] Swiiirlyhunter 0 points 2 points 2 points (+2|-0) ago
They run it 40 times. Anything after 30 is amplified to the point of junk.
[–] AssumingControl ago
40 times refers to the number of duplication cycles during the Reaction. You first have a sample, wash it, purify it and add chemicals to multiply the DNA strands you are looking for. Since DNA is of very low concentration in the sample, you gotta make more of it to have detectable amounts in your end product. Each of the 40 cycles effectively doubles the amount of DNA, because during one cycle one strand of DNA gets split and the halves (mono-strands) are each completed using free bases which you added during preparation.
[–] Swiiirlyhunter ago
Lol, I know..
[–] AntiMarx ago
So riddle me this, call it holocaust maths round two:
How long does preparing the samples take?
How long does cycling samples take?
How long does testing the result of PCR take?
How long does it take untrained workers to do 6 million of these?
TBH maybe this comnent needs its own thread...