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[–] klobos ago 

Technically, yes, but it would be a near impossibility or take millions of years. Take plants genetically modified to resist Roundup. Without the plants being exposed to that chemical the plants that are immune to Roundup would not have any genetic advantage. Meaning that trait would not be necessary for survival and could easily die out in a generation or two. What you would need to do is spray every plant with Roundup and see which ones,if any survive. Then you could breed that plant. Although at that point you are left with only one strain, and you better hope that nothing else threatens that species.